Ashish Kale¹, Ashwini Kale¹

Received: 17 March 2022 / Accepted: 22 August 2022
© Federation of Obstetric & Gynecological Societies of India 2022

Abstract

Aim  To evaluate the oocyte retrieval rate and blastocyst formation rate with DuoStim protocol in patients belonging to POSEIDON groups 3 and 4.

Methods This observational, retrospective, single-center study including 90 patients belonging to POSEIDON groups 3 and 4 was conducted at a tertiary care hospital from October 2017 to March 2020. Patients were allocated into two groups based on POSEIDON classification criteria: group A (POSEIDON group 3) and group B (POSEIDON group 4). DuoStim protocol was performed with human menopausal gonadotropin (hMG) at 225 IU and 300 IU in groups A and B, respectively. Study groups were again subdivided by considering the phase in which stimulation had been done [follicular phase stimulation (FPS) and luteal phase stimulation (LPS)], and then, inference was made accordingly in terms of oocytes retrieval rate and blastocysts formation rate. Data were compiled and analyzed using statistical software SPSS version 20.

Results The baseline characteristics of two groups were compatible with POSEIDON groups 3 and 4. A significant difference was found between study groups with respect to age and anti-mullerian hormone levels (p < 0.05). Significantly, a greater number of oocytes and blastocysts were obtained in LPS stage, substantially more in group A (3.69 ± 3.4 vs. 4.52 ± 4.3 and 1.36 ± 0.65 vs. 3.17 ± 1.84) than group B (2.2 ± 1.36 vs. 3.6 ± 4.5 and 0.41 ± 0.8 vs. 1.29 ± 2.04). A greater blastulation rate (50 vs. 66.7% and 33.3 vs. 50%) and 100% oocyte maturity rate were observed in LPS stage of both the study groups.

Conclusion  In patients belonging to POSEIDON groups 3 and 4, the number of oocytes retrieved and blastocyst formation rate were greater in LPS stage when compared to FPS with DuoStim protocol.

Keywords Luteal phase · Oocyte retrieval · Follicular phase · Cryopreservation · Reproductive technology

Ashish kale is a Director, Department of Obstetrics and Gynaecology, Ashakiran Hospitals and ASHA IVF Centre, Pune, Maharashtra, India, and Ashwini Kale is an Consultant, Department of Obstetrics and Gynaecology, Ashakiran Hospitals and ASHA IVF Centre, Pune, Maharashtra, India.

Ashish Kale drashishkale1978@yahoo.com
Ashwini Kale ashwini13@rediffmail.com

¹Department of Obstetrics and Gynaecology, Ashakiran Hospitals and ASHA IVF Centre, #555, Narayan Chambers, Opposite Narayan Peth Police Station, Pune, Maharashtra 411030, India